Moringa as a household water purification method – community perception and pilot study in Guinea-Bissau | BMC Public Health | Full Text

2022-10-25 06:38:36 By : Mr. Jack Lin

BMC Public Health volume  22, Article number: 1953 (2022 ) Cite this article

Public perceptions of water-related issues are still under-researched topics. The current paper intends to explore a local community’s perceptions regarding household water purification (HWP) strategies, namely before and after trying a new method: moringa seeds powder (moringa-teabag). Flocculant Chemical

Moringa as a household water purification method – community perception and pilot study in Guinea-Bissau | BMC Public Health | Full Text

In September 2020, six focus group discussions (N = 65) assessing perceptions about the usefulness of Moringa oleifera Lam (Moringaceae) as a HWP method (before moringa-based HWP trials), and questionnaires (N = 104) evaluating successes and identifying difficulties (after one week of moringa-based HWP trials). Participants were all women aged over 18 years, living in Ondame, Biombo region, Guinea-Bissau. Data were analyzed using qualitative and quantitative approaches.

The focus group discussions revealed that people are aware of the fact that water can transmit diseases. Although certain persons showed concern about shallow well water safety, people generally underestimate the risk, as they trust tubewell water. Not everyone had an understanding of what water contamination is, or the concept of medical importance. Some respondents declared they use traditional methods such as boiling and bleach to treat water before drinking. However, those who reported no kind of treatment indicated reasons such as lack of time, cost, and bleach’s taste and smell. In the questionnaire, more than half of the participants (68%) reported treating water before consumption. Nevertheless, these results are not consistent with our field notes. Participants demonstrated a strong belief in the capacity of moringa-teabags to purify water and even consider them better or much better (81%) than other methods. Participants asked for more information on moringa-teabag for household water purification.

More information on water treatment and water safety would help to raise public awareness about waterborne diseases. These findings could be used to promote greater adherence to moringa-based HWP as an alternative to household water treatment.

Safe and readily available water is a public health priority, whether it is used for drinking, domestic use, food production, or recreational purposes [1]. Historically, Sub-Saharan Africa experienced recurrent epidemics of waterborne diseases such as cholera and other diarrheal diseases [2,3,4]. In Guinea-Bissau, West Africa, outbreaks of cholera are common, although they have not been officially documented since 2013 [5, 6]. It is estimated that 82% of water for domestic use (drink, bath, and cooking) in the country presents fecal contamination, indicated namely by Escherichia coli [7]. Residents in rural areas seek alternatives to absent water systems, such as tubewells and shallow wells, to meet daily demands of quality water. A shallow well consists of a hole (≈ 200 cm in diameter) that has been dug, bored, driven, or drilled into the ground to extract water [8]. A tubewell (also called a borehole) consists of an iron pipe (15 cm in diameter), bored into an underground aquifer to extract water using a hand pump [9]. These two types of water sources are often contaminated due to the unsafe distances between groundwater-based water wells and pit latrines, frequently found in these rural areas. The engagement of local communities in prevention practices, namely household water purification (HWP), and water, sanitation, and hygiene (WASH) are crucial to avoid waterborne diseases. Examples of effective HWP techniques are bleach (i.e., addition of diluted chlorine solution to disinfect water), or water boiling. Although such methods are employed, their quality and reliability are uncertain, as HWP practitioners remain exposed to waterborne pathogens at home. This may be due to the low effectiveness of those methods, as well as to the consistency of their use (also mentioned in the literature as adherence or compliance). Other drawbacks of these practices have been documented such as the high costs of finding fuel and boiling and cooling water, the risk of burns from cooking fires among young children in developing countries [10], indoor air pollution and acute respiratory infections [11], the contribution of black soot from cooking fires to greenhouse gases [12], and lack of residual protection against recontamination of water during storage [13]. Moreover, chlorination is associated with chlorine taste and smell, and also with the inaccessibility of this treatment product [14].

Moringa oleifera Lam (Moringaceae), hereafter referred as moringa, has recently been recommended for water treatment [15], especially in developing countries, since other chemicals used in water purification are expensive [16]. Due to its numerous properties, this plant has several applications, for example in pharmaceutical, cosmetics, and food nutrition industries. For many years, several studies have been investigating the potential of its seeds and seed husks in drinking water treatment [17,18,19]. Coagulation/flocculation is the most investigated water purification process involving the use of moringa seeds. Evidence shows that it is a natural coagulant, highly efficient, cheap, and environmentally friendly. Recently, studies have been increasingly intensified mainly to understand how the coagulation mechanism occurs, to identify the proteins that are coagulating agents, and to develop isolation techniques [19, 20]. New methods, such as functionalization with nanoparticles [19], have been explored to enhance the removal of contaminants in coagulation/flocculation.

A considerable amount of research has focused on attempting to understand how water quality is perceived by the communities [21]. Findings suggest the need to effectively involve communities at important stages of implementation to ensure the long-term success of water quality interventions, due to their inability to recognize its benefits for health. This is further aggravated by unwillingness to pay for intervention maintenance, faulty perceptions on water treatment, lack of knowledge about health hazards associated with drinking unsafe water, false sense of protection from locally available water, resistance to change of taste or odor of water, and lack of support from male members of the household [22,23,24]. Evidence has been revealing other determinant factors for health-related behavioral change namely how individuals perceive their health risk (risk perception), the effectiveness of the proposed behavior (action outcomes), and their own facilities/ability to perform it (self-efficacy), among others [25,26,27]. The Essential-Perception analysis (EP-analysis) identified five essential topics for individuals adhering to a new health-related behavior (built for dengue prevention) that, when considered as a whole, revealed a correlation with the practices adopted [28, 29]. The present study adopted the rationale of EP-analysis to the prevention of waterborne diseases, aiming to assess essential/key perceptions for community engagement with moringa-based HWP. The current paper intends to explore those key perceptions of a local community in rural Guinea-Bissau regarding HWP strategies, namely before and after trying moringa-teabags as HWP. This will indicate whether the community is or not ready and responsive to these promising methods. Moreover the discrimination of which key perceptions are or not well consolidated within the community can support the selection of messages that need to be spread among the community if a moringa-based HWP is promoted in their area. The approach was based on the relationship between household water purification and household characteristics (such as age and literacy).

The research comprised two approaches: (i) a qualitative analysis using focus group discussions (FGD) before the moringa-based HWP trial (i.e., the participants had no previous experience with moringa-based HWP), to disclose perceptions on HWP strategies, including with moringa, and (ii) a quantitative analysis using a one-to-one questionnaire assessing perceptions regarding moringa as a HWP, following a one-week moringa-based HWP trial. FGDs provided the participants the opportunity to expand on any ideas, not offered in the quantitative questionnaire, that are critical to identify unsuspected obstacles to moringa-teabag use; in turn, the questionnaire allowed an objective assessment of the adherence to the proposed trial and the related perceptions [30].

The study was conducted in Ondame (11°46′N, 15°56′W), a rural area in the Biombo region of Guinea-Bissau. Two reasons supported the selection of Ondame: (i) absence of a water supply system, (ii) geographical proximity and similarity to Quinhamel (rural area) – whose water quality data was previously reported, suggesting a high risk of fecal contamination in drinking water in Ondame [6], concerning epidemiologic data [5], type of water sources, ethnic diversity, and climate. Quinhamel was not chosen as the study area, because the drinking water quality was analyzed there and the topic was familiar to the residents, which could influence the results of the enquiries in the present study. To calculate the sample size, the Epitools platform was used – assuming an infinite population (since the authors could not count the population and no recent official data are available concerning individuals aged over 17 years) and that 90% of the villagers use moringa, a precision of the estimate of 5%, and a confidence level of 90%, a sample of 98 participants was obtained to which about 20% was added to fill a gap, in a total of 120 individuals in the sample. A minimum of 6 FGDs was assumed (one per village), and additional sessions would be planned if data saturation was not achieved. Only one FGD was allocated to each village in order to avoid contact between participants from different FGD; the physical distance between villages (more than 5 km) acts as a buffer. In the villages closest to each other (Dorse and Quinsana, Blom and Blim Blim, Quita-a and Sidja) all the FGD sessions took place on the same day. Three community health workers were involved in the process and established the first contacts with the community leaders of each village, as they are key stakeholders who directly oversee the functioning and health issues in all communities. Participants’ selection for the FGDs was proposed by the leader of each community by indicating around 20 women per village according to the study’s guidelines: women at least 18 years-old, in charge of the drinking water at home. Out of those 20 women, twelve were randomly selected to participate in each FGD, as recommended in the literature [31,32,33] and taking into account the possibility of withdrawals. For the questionnaire, the same sample was used, including both those who participated and those who did not participate in the FGDs, totalling 104 participants.

Six FGDs were conducted and audio recorded, to qualitatively assess communities’ perceptions. Participants were all at least 18 years-old. Each FGD lasted 30 to 45 min and written transcripts of the responses to the various questions were made (in addition to the audio-recordings). FGD sessions were conducted in a roundtable format gathering a minimum of 4 and a maximum of 12 individuals, as recommended [31,32,33]. Selected topics were discussed with a moderator until data saturation was reached or no new information was uncovered, in a local language (Guinea-Bissau creole). The moderator encouraged an open discussion, hoping that group dynamics would reveal deep feelings and thoughts. An FGD guide was used to direct the discussion (see supplementary material) containing a set of open-ended questions exploring various aspects such as the effects of unsafe water on health (e.i, are there diseases that are transmitted by water, or not?). The FGD guide was previously tested in a village that did not participate in the main study (Ondame) and modified according on the obtained feedback. The fieldworker was present as a facilitator during the meetings and interviews and took notes to document participants' comments relevant to the qualitative analysis. No information that could identify participants was retained in the transcription, the analysis or the subsequent report of findings.

FGD participation’s informed consent was given verbally and audio-recorded as part of the discussion since the level of literacy of the participants is very low. Participants in FGDs were asked confidentiality about all that was discussed in the sessions. The meetings were held at a time and place convenient to the participants, i.e., mostly in the mornings and in a village’s community hall. All participants volunteered to join the study. At the end of each session there was a socializing party, and this gathering of the participants was encouraged by the research team. The study was approved by the ethics committee (090/CNES/INASA/2020) from the National Institute of Public Health (INASA), Guinea-Bissau.

The Essential-Perception analysis (EP-Analysis) was validated in the dengue context, revealing a correlation between having the essential perceptions and adhere to the proposed preventive behavior (those selected assessed perceptions were thus considered essential for that behavioral adherence). In this study the Essential Perceptions Framework was adopted and adapted to the waterborne disease setting [28]. Following this methodology, five essential topics were maintained (Medical Importance, Local Risk perception, Household Water Purification, Water Contamination, and moringa-based—new HWP method) and 13 adapted essential perceptions (Table 1) were addressed based on the FGDs guide. The study was conducted from late September to the beginning of December 2020.

A post-test-only design was performed, to assess perceptions one week after the moringa-based HWP trial. The fine moringa seed powder (moringa-teabag) was previously produced by Diocesan Caritas of Bissau. Then, moringa-teabags were prepared by us, each containing 2 g of moringa seed powder, to be diluted in 20 L of water (i.e., in a proportion of 100 mg/L) for 5 min, which has the potential to eliminate 99.9% of several pathogenic bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus (MRSA) [34].

Each participant received seven moringa-teabags to use for one week, and the questionnaire was presented in the following week (late November 2020).

Questionnaires applied through one-to-one interviews were then used to extract in-depth information from 104 participants (65 from FGD plus those who did not have the opportunity to participate in the FGD) in Ondame’s villages. A semi-structured format was employed to collect data, exploring various aspects such as their belief in moringa's capacity to purify water (e.i., what was the general impression of using the moringa-teabags for water treatment?). Participation in the face–to–face questionnaire was based only on verbal consent since the level of literacy is very low. The surveys were performed by trained personnel (leading researcher) during October and beginning of November 2020. The study was approved by the ethics committee (090/CNES/INASA/2020) from the National Institute of Public Health (INASA), Guinea-Bissau.

The topics addressed concerned: General perception, Water sensory evaluation, Moringa adherence, and Comparison between moringa-based and other HWP (Table 2).

Researchers coded the obtained data, as it is the most appropriate strategy to analyze FGD data [35], and then extracted it from the transcripts to conduct further analysis. The analysis was both deductive, when considering the previously defined topics (displayed in Tables 12), and inductive, when categories/topics emerged purely from the data. The research team critically reviewed each analysis product, ensuring analytic rigor and reliability by confirming that the data and illustrative excerpts were extracted to the correct domain. Discrepancies in data interpretation were discussed and resolved via consensus.

After the survey was completed, data was transferred to a secure server and a descriptive statistical analysis was carried out using SPSS v. 27.0 program SPSS Statistics software (IBM Corp., Armonk, NY, USA). A chi-square (χ2) and binomial tests [36,37,38] were applied to identify household characteristics leading to HWP acceptance and test dichotomous variables respectively. For all analyses, the level of significance was set at 5% (p ≤ 0.05).

The socio-demographic characteristics of the sample are described in Table 3. Of the 110 submitted questionnaires, a total of 104 fully completed ones were returned and analyzed. The remaining 6 questionnaires were not fully completed and were discarded from the analysis. Adding to the previously established ones, a new topic/category emerged from the data during FGDs: the main source of water (Table 3). Findings from both qualitative and quantitative analysis are presented below by topic.

Out of those 65 had previously participated in the FGDs sessions, comprising an average of ten participants (min. = 4, max. = 12). Demographic information concerning the respondent community is given in Table 3.

Participants showed awareness of the medical importance of waterborne diseases: they are conscious that water can transmit diseases and some were able to give a correct description of a waterborne disease. For example, “some bacteria can be in the water and you do not know and you are going to drink that water and you get sick, but if you drink good water, you will not get diseases” (FGD1, Int 3). Those who knew an example of waterborne diseases described “diarrhea, vomiting and many diseases in the water” (FGD3, Int 12). However, others were unable to name a correct example of waterborne disease “well I do not know, it is you who will tell us (FGD3, Int 9)”. Regarding mortality/morbidity, participants did not recognize the severity of waterborne diseases. They perceive the loss of family members due to illness, although they are not able to identify if that was due to water consumption, and in some cases they do not even know what the cause was – “we may have lost family members and we do not know that it is because of the water, except when it was a cholera outbreak, then we knew that it was because of the water” (FGD1, Int 13). On the other hand, they were aware that diarrhea is a sign of illness and that it can cause damage to our health – “diarrhea is a very big disease” (FGD1, Int 16).

The perception of water quality in Ondame varies according to the type of water source (tubewell or shallow well). Ondame’s tubewell water is perceived as cleaner and safer than shallow well water – “the water from tubewell is better than from the shallow well, yes, because it is clean, while the water from the shallow well is dirty” (FGD2, Int 20). One participant declared having no concern at all with the tubewell water – “I have no concern with tubewell water and as we now have it within our reach and we are going to get it, and we spare the tiring boiling” (FGD1, Int 21). Despite confidence in the safety of their water, namely from tubewells, some participants were aware of its potential contamination with rainwater – “when it rains that cloudy, red water will go into the well” (FGD4, Int 28). Yet, some raised concerns about the shallow water – “when you are seeing an open shallow well water without lid, you know right away that it is not clean” (FGD1, Int 23). Others consider that they drink shallow well water due to the lack of alternatives, even though they are aware that it can be dangerous (contaminated). Although some participants recognize a higher risk in shallow wells, some believe it is safe since their ancestors already drank that water. A participant shared her thoughts: “you cannot say that this water we drink is not good, because since we were born, this is the water that we have been drinking, as we have not found any other pump (tubewell) here and this is the one we live with” (FGD4, Int 18).

Not all participants revealed an understanding of what water contamination is – “I do not know” (FGD6, Int 87), and some defined it as “contaminated water, it is dirty water that is contaminated water” (FGD5, Int 84). Nevertheless, they perceive contamination more as a transmission mode from person to person – “contamination is when I have diarrhea and vomiting and if a get visits from my colleague, he/she can get sick through my illness” (FGD3, Int 86). Participants correctly identify rainwater as the main cause of contamination – “when it rains that cloudy, red water will go into the well” (FGD4, Int 96).

In FGDs, a wide variety of comments arose regarding HWP. Respondents commented that they already use some traditional methods to treat water before drinking it – “by bleach, or boiling or even lemon drop, or another if you have” (FGD3, Int 47). Participants perceive that treating water at the home level is important and reported that they do it – “I use a cloth to filter, when I cannot filter, I normally put a drop of bleach” (FGD6, Int 41). For those who do not treat the water, the reason is that they feel safe – “before we had the tubewell, we used to filter the water, but after there was the tubewell, I just wash the basin/bowl, clay pot, and lid very clean, and remove the water and put it into a clay pot and cover it with cloth” (FGD3, Int 36).

Although participants mentioned that they treat water, they pointed out the barriers such as price, smell, time, and taste – “As we do not have time for that, while we are working in the fields, we drink from shallow” (FGD4, Int 52); another participant reported that “some do not like to use bleach because of the smell, that is why they do not use it” (FGD6, Int 55). In addition, one participant said: “In fact it is very difficult for anyone here to put bleach in the water, it is just because of this quarantine (covid-19) that has arrived that people are now putting bleach in, if it was not for this it is difficult to see anyone putting bleach in the water” (FGD6, Int 56). Participants perceive different needs to treat water according to their water source and aim. For drinking, they collect from tubewell, whilst a shallow well is used for cooking or bathing. The overall impression the participants gave was the need for a tubewell – “if there is a tubewell, we avoid all that, it is just going and getting the water as it is not necessary to filter, but if you are tired and arrive at night and you are thirsty, you will get the water and drink it without filtering” (FGD4, Int 58).

Participants demonstrated knowledge about moringa, but in terms of food and medicinal aspects. They appeared to be surprised about its use as an alternative for traditional HWP, as they had never heard of it – “yes, it is a surprise, as we have never done it and we do not even know about it and we have it here in abundance” (FGD1, Int 73). They showed great readiness to accept the moringa-teabag as an alternative for HWP water purification at the household level – “yes, we will because you have shown us that it can remove the bacteria” (FGD2, Int 81). Several participants were very positive about their ability to carry out the process of using the moringa-teabags. They were interested in sharing information regarding the process, claiming that the main difficulty might be to implement the process. The request for information was consistent in all the groups – “just tell us, if you explain to us and if it is something to help us, we will do it because what we want is to get rid of these diseases” (FGD1, Int 80). There was also great interest in having a moringa-teabag to test the method – “I think everyone will accept it because if there was no interest, we would not be here” (FGD2, Int 76). Further, one participant illustrated that its future use would depend on the results from the first uses – “if you bring it to us once and we feel it is good, even if you do not bring it anymore for us, we will keep doing it because we have it here” (FGD4, Int 79). The examples of relevant citations for each topic of interest can be seen in the supplementary material (Tables S1 and S2).

Over half of the households (68%) treat their drinking water before consumption; more than a quarter (32%) do not. The majority of the participants considered the tubewell as the main water source for drinking and other household uses (Fig. 1).

Percentage distribution of responses to questions concerning the purification of drinking water (A) and the main source of drinking water (B)

Belief in moringa can be defined by three broad categories: nutritional, medicinal, and chemical aesthetic attributes. In this study, the focus was on the chemical aesthetic attributes, and the participants were asked about their general impressions after using the moringa-teabag. Overall, households (93%) indicated that they were “Satisfied”, “Slightly satisfied” or “Extremely satisfied” with this water treatment(Fig. 2) However, 8% of households indicated that they were “neither satisfied nor dissatisfied" or "moderately dissatisfied”.

Percentage (%) of respondents' answers concerning overall impression about moringa-teabags

Figure 3 shows that 79% of the households used the teabag in all the water consumed. Among those who did not use it, 48% said they did not have enough, while 22% reported headaches after the first use, and 13% reported lack of time.

Percentage distribution of responses to questions concerning the use of moringa-teabag in all the water consumed (A) and the reason for not using it (B)

Respondents were asked to evaluate the quality of the moringa-teabag treated water, based on two sensory characteristics (color and taste). More than half of the households (55%) indicated that their drinking water color was changed; among these, 93% said the water became cloudy. Around 65% of the participants reported that the water taste was changed; it tasted either sweet (89%), sour (8%) or salty (3%) (Fig. 4).

Percentage distribution of responses to questions concerning water sensory characteristics: A: change in color, B: change in taste

Respondents were asked to rate the importance of using moringa-teabags for HWP, how easy/difficult was it, and what type of difficulties were found. The majority (95%) strongly believed that using moringa-teabag for HWP is very important or quite important, while the remainder said it is somewhat important or not important (Fig. 5). Most participants (92%) assertively mentioned that it was very easy or easy to use, in contrast with 7% that were uncertain, saying that it is neither easy nor difficult, and 1% for whom it was hard. The results show that the main difficulty found was related with measuring the amount of water for each teabag (20%), and psychological barriers (2%). When asked about the possibility of using the moringa-teabag in the future to treat water at home, 94% of the participants answered "yes" and 6% "no". For the latter, the main reason was that they did not like it (75%) or because it changed the taste of the water (25%). Regarding recommendation of the teabag as HWP, more than half (94%) said "definitely yes" or "yes"; 6% said "no" (Fig. 5).

Percentage distribution of responses to questions concerning general appreciation (A), ease/difficulty to use the moringa-teabag (B), experienced difficulties (C), recommendation to others (D), willingness to use in the future to purify drinking water (E) and, if not, why (F)

Data on HWP practices were derived from three main core questions in the household survey (do you use other treatment methods, how does it compare with the use of the moringa-teabag, and would you recommend moringa-teabag to others). The findings show that (Fig. 6), among those who reported treating water for drinking, bleach was the predominant HWP method, used by over one-third of the surveyed households (49%), followed by filtering through cloth (39%) (Fig. 6). Other practices such as boiling or lemon juice drops were less common. Comparing moringa-teabag with other traditional HWP methods, the majority (81%) said that the former is better or much better than other methods, and 16% stated it is similar. Few households reported that moringa-teabag is worse (3%).

Percentage distribution of responses to questions concerning the comparison of moringa-based with other HWP: use of other methods (A), if yes, which ones (B), and which one is best (C)

The relationship between household water purification and household characteristics was analyzed using chi-square tests (χ2), while a binomial test was carried out to test dichotomous variables.

Research Hypothesis 1—The proportion of people who purify water before drinking equals that of people who do not (H0); H1—The proportion of people who purify water before drinking is different from the proportion of people who do not. According to the results, the null hypothesis cannot be rejected (P > 5%) and it is concluded that the proportion of those who purify water before drinking equals that of those who do not (Table 4).

Research Hypothesis 2—The proportion of people who use water as soon as it is collected equals that of those who do not (H0); H1—the proportion of people who use water as soon as they collect it differs from that of people who do not do that. The results showed that the null hypothesis is rejected, so we can conclude that there is no difference between the two groups (Table 4).

Research hypothesis 3 (relation of water treatment with schooling).

There is no difference between high schoolers and non-high schoolers regarding water purification practices (H0); H1—there is a difference between high schoolers and non-high schoolers regarding water purification practices. There was no difference between the two subgroups (P = 0.70) and, therefore, the literacy level does not influence the procedures concerning the purification of water before its consumption (Table 5).

Research hypothesis 4 (relation of water treatment practices with age).

There is no difference between young adults and adults regarding water purification (H0); H1—there is a difference between young adults and adults regarding water purification. There was no difference between the two subgroups (P = 0.463) and, so, age did not influence the practices concerning the purification of water before its consumption.

A first analysis indicated some influence of age (years), with young people tending to purify water before consumption, but with a very tight statistical significance (P ≤ 0.05). In a second analysis, by considering two broad age groups (young adults and adults), the null hypothesis can be rejected with much more confidence (Table 5).

Qualitative and quantitative methods employed in this study allowed to gain insight into people's perceptions regarding the health effects of drinking water and household water purification practices in rural Guinea-Bissau households. Both approaches helped to identify key factors that influence the acceptance of the proposed methods, the water quality and its sustainability. Most traditional HWP methods (such as bleach or boiling) in the study area are reactive (in response to governmental announcements) rather than proactive (autonomous and well-planned by communities).

Several studies have confirmed that water conservation attitudes (WASH) and behaviors are closely related [39, 40]. The presented study indicates that the participants' belief in the medical importance of water is often biased. The nature of this bias seems associated to a ‘‘risk perception bias’’ and was noticed in participants who had some form of broad knowledge that contaminated water could cause health problems, and thus be more likely to find and report waterborne diseases. This was notorious in the case of a participant who presented a certain knowledge, given that her partner is a nurse.

For most of the participants, the concept of medical importance might not have been well understood. However, they know that water is a route for disease transmission, although few can name such diseases. They recognize the loss of a family member but do not know if it related to a waterborne disease, or to which one. As in the study carried out by Nazareth et al. [29] on the impact of a dengue outbreak on the preventive perceptions of the community from a temperate region (Madeira Island, Portugal), the participants did not show any perception of the medical importance.

The risk of well water differs, depending on the type of source (tubewell or shallow well). In general, respondents perceive that Ondame's tubewell water is safe, and show a high appreciation for tubewell water quality. Similar perceptions have been reported by Wu et. al [41] in their study on the impact of tubewell access and tubewell depth on childhood diarrhea in Matlab, Bangladesh. The natural filtration and the tubewell structure associated with groundwater supplies could be the main reason for the participant’s confidence in the safety of tubewell water. Drinking water stakeholders (companies, governments, and WHO/UNICEF) in Ondame (Guinea-Bissau) should inform the community about water quality issues such as safety and risks. Effective risk communication is complex and requires a thorough understanding of the information needs and risk perception of the general public [22, 42].

Household water purification is an important and effective part of the multi-barrier approach to ensure water quality and safety [43, 44]. Participants in FGDs declared they treat the water for drinking. However, this is not consistent with our field notes and even with some observations of the participants in FGDs who reported that they only treat water when they are recommended by community health workers. But, on the other hand, these results were in line with those from the questionnaire, where 68% of the participants reported using other methods to purify water at the home level. This difference in results could be explained by the possibility that participants consider cloth filtration as an effective treatment method. It is important to mention that from our observations and field notes, and even though they consider tubewell water safe, they normally use cloth to filter the water before putting it into the clay pot. Nevertheless, Roche et. al [45], studying public perceptions and improved water safety, reported that the respondents treated their drinking water using an in-home treatment method such as a filter. In FGDs, many participants indicated barriers to HWP practices. Sensory characteristics such as the smell and taste of bleach, and lack of time and money were the main reasons for not treating water at home.

In the questionnaire, the change in color or taste was also evidenced, although most participants considered that water’s taste improved after the use of moringa-teabag. A variety of treatment methods was reported, with almost half of respondents treating water with bleach followed by cloth filtering, or using more than one method. This result (cloth filtering) is also found in other studies on households depending on water wells (tubewell and shallow wells) [46,47,48]. However, the effectiveness of these treatments to reduce or eliminate microbiological hazards in water is questionable. The hypotheses tests showed that neither age nor literacy level influenced the water treatment practices in Ondame. This could be related to cultural issues (bias), such as the fact that they believe their grandparents drank this water and nothing bad happened to them.

Concerning moringa-teabag application, most participants believe in its capacity to purify water and show great willingness to use it for this purpose, considering it even much better than other methods and definitely recommending others to use it. This belief is supported by their knowledge of moringa as a medicinal and nutritionally valuable plant [49]. It should be noted that although there is a great belief in the moringa-teabag's effectiveness, some participants (30%) advised against it, claiming to have suffered from headaches after the first use, or that they or family members did not like it, or that they did not feel the need to have all their water treated, probably making it difficult to accept by these participants. Complaints about headaches may be linked to the high number and variety of bioactive compounds present in moringa seeds, although there is no evidence in the literature to support this side effect. Few studies on humans have analyzed the health benefits of moringa, but it seems to be well tolerated and to produce no side effects [50, 51].

The teabag for water purification can be an affordable and important practice to reduce waterborne diseases and to improve the quality of life for a large proportion of poor populations [52, 53]. The authors suggest the communication of water contamination levels to the communities [6], to raise their awareness of the actual water quality in their regions. Accurate risk perception can promote adherence to proposed methods of household water purification, such as the moringa-teabag. Another key step could be to involve influential actors, such as religious groups, local entities, and community leaders, in promoting commitment to these preventive practices.

However, it should be noted that during this study there were no outbreaks (history factor) that could jeopardize the study. The fact that respondents were only women (selection factor) does not influence the results, because women are the ones who take care of water at home. The study period (maturation factor) was not long, but the fact that two methodologies were applied and that the FGD participants also participated in the questionnaires may have influenced their acceptance of the use of the moringa-teabag. There was no loss of participants (mortality factor) during this study, but this is not a controllable situation and, over time, mortality might occur.

To sum up, this study provides three innovative pieces of information: i) community perceptions regarding waterborne diseases in Guinea-Bissau, ii) community perceptions regarding moringa-based HWP as a water treatment strategy, and iii) a successful pilot study on moringa use as a household water purification method.

To date, there is no evidence in the literature reporting: i) community perceptions regarding waterborne diseases in Guinea-Bissau, ii) community perceptions on the use of moringa as a water treatment strategy, and iii) studies on actual moringa use as a household water purification method. This makes our findings pioneer/innovative and crucial due to the absence of sustained treated water supply or strategy in countries such as Guinea-Bissau, and to the disadvantages of the more common HWP methods. This study indicates that the participants were willing to try the moringa-teabag as HWP and after having tried it they were very satisfied with the results. Nevertheless, it is apparent that certain beliefs may influence some perceptions, such as the fact that they drink the same water for many years and think it is not contaminated, or the fact that they do not use chlorine because of the smell.

Correct perception of water contamination and risk are the key factors influencing water-use behavior. The majority of the respondents have misconceptions about water treatment, representing a high risk of waterborne diseases.

This paper demonstrates the importance of this new contribution to knowledge, providing detailed information to understand the correct perception of water contamination and risk correlated with water-use behavior. The results of this research can support the implementation of collaborative social innovation in order to understand how moringa seed powder will impact the environment and the social behavior along the water supply chain in rural areas. They can also be seen as new approaches to the practical objectives of identifying main barriers hampering the effective implementation of social innovation, and better understanding how moringa-teabags packaging innovation might impact the environment and various stakeholders across the water quality supply chain, especially as it relates to public health, infrastructure, cleaning of formal and informal water collectors, consumers, and policy.

These findings could be used to inform future campaigns to promote moringa as an alternative for household water treatment, in communities without water supply systems, with the potential for greater community engagement than current HWPs.

Analytic data are available in supplementary file

The National Institute of Public Health

Statistical Package for the Social Sciences

World Health Organization, “Household Water Treatment and Safe Storage Following Emergencies and Disasters South Asia earthquake and tsunami”, 2002. [Online]. Available: www.who.int/water_sanitation_health/hygiene/emergencies/em2002chap7.pdf. Accessed: 16 Jun 2021.

Mengel MA, Delrieu I, Heyerdahl L, Gessner BD. Cholera outbreaks in africa. in: nair g, takeda y (eds) cholera outbreaks. Curr Top Microbiol Immunol. 2014;379:117–44.

Fenollar F, Mediannikov O. Emerging infectious diseases in africa in the 21st century. New Microbes New Infect. 2018;26:S10–8.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Mutono N, Wright J, Mutembei H, Muema J, Thomas M, Mutunga M, Thumbi SM. The nexus between improved water supply and water-borne diseases in urban areas in Africa: a scoping review protocol. AAS Open Res. 2020;3:12.

PubMed  PubMed Central  Google Scholar 

Gunnlaugsson G, Angulo FJ, Einarsdóttir J, Passa A, Tauxe RV. Epidemic cholera in Guinea-Bissau: the challenge of preventing deaths in rural West Africa. Int J Infect Dis. 2000;4(1):8–13.

Article  CAS  PubMed  Google Scholar 

Bancessi A, Catarino L, Silva MJ, Ferreira A, Duarte E, Nazareth T. Quality assessment of three types of drinking water sources in Guinea-Bissau. Int J Environ Res Public Health. 2020;17(9):1–15.

INE and UNICEF. Inquérito aos Indicadores Múltiplos (MISC6). 2020. [Online]. Available: https://mics-surveys-prod.s3.amazonaws.com/MICS6/West and Central Africa/Guinea-Bissau/2018–2019/Snapshots/Guinea Bissau 2018–19 MICS Statistical Snapshots_Portuguese.pdf. Accessed: 15 May 2021.

Rangsayatorn N. Shallow groundwater quality assessment in Muang District. Phayao Naresuan University J. 2006;14(2):1–8.

Luby SP, Gupta SK, Sheikh MA, Johnston RB, Ram PK, Islam MS. Tubewell water quality and predictors of contamination in three flood-prone areas in Bangladesh. J Appl Microbiol. 2008;105(4):1002–8.

Article  CAS  PubMed  Google Scholar 

Gilman RH, Skillicorn P. Boiling of drinking-water: can a fuel-scarce community afford it? Bull World Health Organ. 1985;63(1):157–63.

CAS  PubMed  PubMed Central  Google Scholar 

Smith KR, Samet JM, Romieu I, Bruce N. Indoor air pollution in developing countries and acute lower respiratory infections in children. Thorax. 2000;55(6):518–32.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jacobson MZ. Global direct radiative forcing due to multicomponent anthropogenic and natural aerosols. J Geophys Res Atmos. 2001;106(D2):1551–68.

Mintz ED, Tauxe RV, Reiff FM. Safe water treatment and storage in the home: a practical new strategy to prevent waterborne disease. JAMA. 1995;273(12):948–53.

Article  CAS  PubMed  Google Scholar 

Geremew A, Mengistie B, Alemayehu E, Lantagne DS, Mellor J, Sahilu G. Point-of-use water chlorination among urban and rural households with under-five-year children: a comparative study in Kersa Health and Demographic Surveillance Site, Eastern Ethiopia. J Water, Sanit Hyg Dev. 2018;8(3):468–80.

Alam MW, Pandey P, Khan F, Souayeh B, Farhan M. Study to investigate the potential of combined extract of leaves and seeds of moringa oleifera in groundwater purification. Int J Environ Res Public Health. 2020;17(20):1–13.

Varkey AJ. Purification of river water using moringa oleifera seed and copper for point-of-use household application. Sci African. 2020;8: e00364.

Zakaria, S. N. S., Che Ku Yahya, C. K. M. F., Yahya, N. Y. The potential of Moringa oleifera seeds for fungal disinfection in water. Materials Today: Proceedings. 2022. 57, 1369–1372. https://doi.org/10.1016/J.MATPR.2022.02.585.

Dezfooli SM, Uversky VN, Saleem M, Baharudin FS, Hitam SMS, Bachmann RT. A simplified method for the purification of an intrinsically disordered coagulant protein from defatted Moringa oleifera seeds. Process Biochem. 2016;51(8):1085–91. https://doi.org/10.1016/J.PROCBIO.2016.04.021.

Gomes H de O, Freire P. de T. C, do Nascimento R. F, Pereira Teixeira R. N. Removal of contaminants from water using Moringa oleifera Lam. as biosorbent: An overview of the last decade. J Water Process Eng. 2022;46:102576. https://doi.org/10.1016/J.JWPE.2022.102576.

Ueda Yamaguchi N, Cusioli LF, Quesada HB, Camargo Ferreira ME, Fagundes-Klen MR, Salcedo Vieira AM, Gomes RG, Vieira MF, Bergamasco R. A review of Moringa oleifera seeds in water treatment: Trends and future challenges. Process Saf Environ Prot. 2021;147:405–20. https://doi.org/10.1016/j.psep.2020.09.044.

Shahangian SA, Tabesh M, Yazdanpanah M. How can socio-psychological factors be related to water-efficiency intention and behaviors among Iranian residential water consumers. J Environ Manage. 2021;288: 112466.

De França DM. Factors influencing public perception of drinking water quality. Water Policy. 2010;12(1):1–19.

Bradford LEA, L. Bharadwaj A, Okpalauwaekwe U, Waldner CL. Drinking water quality in Indigenous communities in Canada and health outcomes: a scoping review. Int. J. Circumpolar Health 2016;75:https://doi.org/10.3402/ijch.v75.32336.

Francis MR, Nagarajan G, Sarkar R, Mohan VR, Kang G, Balraj V. Perception of drinking water safety and factors influencing acceptance and sustainability of a water quality intervention in rural southern India. BMC Public Health. 2015;15(1):1–9.

Brewer NT, Weinstein ND, Cuite CL, Herrington JE. Risk perceptions and their relation to risk behavior. Ann Behav Med. 2004;27(2):125–30.

Mills B, Reyna VF, Estrada S. Explaining contradictory relations between risk perception and risk-taking. Psychol Sci. 2008;19(5):429–33.

Ferrer RA, Klein WMP. Risk perceptions and health behavior. Curr Opin Psychol. 2015;5:85–9.

Article  PubMed  PubMed Central  Google Scholar 

Nazareth T, Teodósio R, Porto G, Gonçalves L, Seixas G, Silva AC, Sousa CA. Strengthening the perception-assessment tools for dengue prevention: a cross-sectional survey in a temperate region Madeira. Portugal BMC Public Health. 2014;14:39.

Nazareth T, Sousa CA, Porto G, Gonçalves L, Seixas G, Antunes L, Silva AC, Teodósio R. Impact of a dengue outbreak experience in the preventive perceptions of the community from a temperate region: madeira island, portugal. PLoS Negl Trop Dis. 2015;9(3):e0003395.

Article  PubMed  PubMed Central  Google Scholar 

Bryman A. Quantity and Quality in Social Research (Contemporary Social Research) Meliora Lamare. 1988. [Online]. Available: https://www.academia.edu/28944371/Alan_Bryman_Quantity_and_Quality_in_Social_Research_Contemporary_Social_Research_. Accessed 18 Jun 2021.

Coenen M, Stamm TA, Stucki G, Cieza A. Individual interviews and focus groups in patients with rheumatoid arthritis: A comparison of two qualitative methods. Qual Life Res. 2012;21(2):359–70.

Liamputtong, P. Focus Group Methodology: principles and practice. In Focus Group Methodology: principles and practice. SAGE Publications Ltd.; 2011. https://doi.org/10.4135/9781473957657.

Kitzinger J. Qualitative Research: Introducing focus groups. BMJ. 1995;311:299.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Virk AK, Kumari C, Tripathi A, Kakade A, Li X, Kulshrestha S. Development and efficacy analysis of a Moringa oleifera based potable water purification kit. J Water Process Eng. 2019;27:37–46.

Onwuegbuzie AJ, Dickinson WB, Leech NL, Zoran AG. A Qualitative Framework for Collecting and Analyzing Data in Focus Group Research. Int J Qual Methods. 2009;8(3):1–21.

Thompson CB. Descriptive Data Analysis. Air Med J. 2009;28(2):56–9. https://doi.org/10.1016/J.AMJ.2008.12.001.

Lee, S. W. (2022). Methods for testing statistical differences between groups in medical research: statistical standard and guideline of Life Cycle Committee. Life Cycle, 2. https://doi.org/10.54724/LC.2022.e1.

Pestana MH, Gageiro JN. Análise de dados para ciências sociais: a complementaridade do SPSS. 4ª ed. Lisboa: Sílabo; 2005. p. 681-683. ISBN 972-618-391-X.

Gilg A, Barr S. Behavioural attitudes towards water saving? Evidence from a study of environmental actions. Ecol Econ. 2006;57(3):400–14.

Koop SHA, Van Dorssen AJ, Brouwer S. Enhancing domestic water conservation behavior: A review of empirical studies on influencing tactics. J Environ Manage. 2019;247:867–76.

Article  CAS  PubMed  Google Scholar 

Wu J, Yunus M, Streatfield PK, van Green A, Escamila V, Akita Y, Serre M, Emch M. Impact of tubewell access and tubewell depth on childhood diarrhea in Matlab, Bangladesh. Environ Heal A Glob Access Sci Source. 2011;10(1):1–12.

Zhang L, Li H, Chen K. Effective risk communication for public health emergency: Reflection on the covid-19 (2019-ncov) outbreak in Wuhan, China. Health. 2020;8(1):64.

Plummer R, Velaniškis J, de Grosbois D, Kreutzwiser RD, de Loë R. The development of new environmental policies and processes in response to a crisis: the case of the multiple barrier approach for safe drinking water. Environ Sci Policy. 2010;13(6):535–48.

Minnes S, Simpson H. Capacity for source water protection in Ontario’s privately-serviced rural areas. Geoforum. 2020;116:12–23.

Roche SM, Jones-Bitton A, Majowicz SE, Pintar KDM, Allison D. Investigating public perceptions and knowledge translation priorities to improve water safety for residents with private water supplies: a cross-sectional study in newfoundland and labrador. BMC Public Health. 2013;13(1):1225.

Article  PubMed  PubMed Central  Google Scholar 

Mwabi JK, Adeyemo FE, Mahlangu TO, Mamba BB, Brouckaert BM, Swartz CD, Offringa G, Mpenyana-Monyatsi L, Momba MNB. Household water treatment systems: A solution to the production of safe drinking water by the low-income communities of Southern Africa. Phys Chem Earth. 2011;36(14–15):1120–8.

Abubakar IR. Understanding the socioeconomic and environmental indicators of household water treatment in Nigeria. Util Policy. 2021;70(101209):1–10.

Bancessi A, Bancessi Q, Baldé A, Catarino L. Present and potential uses of Moringa oleifera as a multipurpose plant in Guinea-Bissau. South African J Bot. 2020;129:206–8.

Awodele O, Oreagba IA, Odoma S, Da Silva JAT, Osunkalu VO. Toxicological evaluation of the aqueous leaf extract of Moringa oleifera Lam. (Moringaceae). J Ethnopharmacol. 2012;139(2):330–6.

Monera-Penduka TG, Maponga CC, Wolfe AR, Wiesner L, Morse GD. Effect of Moringa oleifera Lam. leaf powder on the pharmacokinetics of nevirapine in HIV-infected adults: A one sequence cross-over study. AIDS Res Ther. 2017;14:12.

Article  PubMed  PubMed Central  Google Scholar 

Avilés-Gaxiola S, León-Félix J, Jiménez-Nevárez YB, Angulo-Escalante MA, Ramos-Payán R, Colado-VelázquezIII J, Heredia JB. Antioxidant and anti-inflammatory properties of novel peptides from moringa oleifera lam. Leaves South African J Bot. 2021;141:466–73.

Bancessi A, Pinto MMF, Duarte E, Catarino L, Nazareth T. The antimicrobial properties of Moringa oleifera Lam. for water treatment: a systematic review. SN Appl Sci. 2020;2:323.

George TT, Obilana AO, Oyenihi AB, Rautenbach FG. Moringa oleifera through the years: a bibliometric analysis of scientific research (2000–2020). South African J Bot. 2021;141:12–24.

The authors wish to the on-site logistic support of Caritas Diocesana de Bissau (Monica Canavesi). We thank the entire Local health community workers, who facilitated contact with the local population. To Eng. Quintino Bancessi for providing transportation during fieldwork and also for all his work as a facilitator.

This study was funded by the Foundation for Science and Technology (FCT, Portugal) through the PhD grant SFRH/BD/135356/2017, the research centers’ grants UIDB/00329/2020 (cE3c), UID/AGR/04129/2019 (LEAF).

Nova School of Business and Economics, Nova University of Lisbon, Campus de Carcavelos, Rua da Holanda, n.1, 2775-405, Carcavelos, Portugal

Unidade Clínica Tropical, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, R. da Junqueira 100, 1349-008, Lisbon, Portugal

Centro de Malária e Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, R. da Junqueira 100, 1349-008, Lisbon, Portugal

Department of Sciences and Engineering of Biosystems, Institute of Agronomy, University of Lisbon, Tapada da Ajuda, 1349-017, Lisbon, Portugal

Universidade Jean Piaget Guiné-Bissau, Campus de Antula, Bissau, Guinea-Bissau

Centre for Ecology, Evolution and Environmental Changes (cE3c), & Global Change and Sustainability Institute (CHANGE), Faculty of Sciences, University of Lisbon, Campo Grande, 1749-016, Lisbon, Portugal

Aducabe Bancessi & Luís Catarino

Unidade de Parasitologia Médica, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, R. da Junqueira 100, 1349-008, Lisbon, Portugal

Faculdade de Medicina, Universidade Católica Portuguesa, Campus de Sintra, Estr. Octávio Pato, 2635-631, Rio de Mouro, Portugal

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Conceptualization, Aducabe Bancessi, and Teresa Nazareth; methodology, Aducabe Bancessi, Teresa Nazareth and Rosa Teodósio; Investigation, Aducabe Bancessi and Teresa Nazareth; resources, Teresa Nazareth, Elizabeth Duarte, Aladje Baldé and Luís Catarino; writing—Original Draft, Aducabe Bancessi, Luís Catarino, Rosa Teodósio, Elizabeth Duarte and Teresa Nazareth; writing—review and editing, Aducabe Bancessi, Elizabeth Duarte, Luís Catarino, Rosa Teodósio, and Teresa Nazareth; supervision, Elizabeth Duarte, Luís Catarino, Rosa Teodósio, and Teresa Nazareth. All authors have read and agreed to the published version of the manuscript.”

The ethics committee of the National Institute of Public Health (INASA), Guinea-Bissau approved the study protocol (090/CNES/INASA/2020). Informed consent was obtained from all participants. All methods were performed in accordance with the relevant guidelines.

The authors have no conflicts of interest to report.

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Bancessi, A., Teodósio, R., Duarte, E. et al. Moringa as a household water purification method – community perception and pilot study in Guinea-Bissau. BMC Public Health 22, 1953 (2022). https://doi.org/10.1186/s12889-022-14344-w

DOI: https://doi.org/10.1186/s12889-022-14344-w

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